Tasmania has a strong record of successful in situ plant conservation but there will always be a role for the integration of various ex situ measures into a plant conservation program due to pressure by threatening processes on wild populations. This paper replaces a 15 year old strategy for ex situ conservation in Tasmania. Progress in ex situ measures for Tasmanian plants is described and broadly evaluated against the previous strategy. Rare and threatened species are considered to be a high priority group for resources if intensive management is required. Endemic species likely to be adversely impacted by climate change would be a high priority for ex situ conservation. Seed banking to capture as much genetic variation in these species is suggested. Eight ex situ methods are briefly described and their application in Tasmanian instances noted. The Tasmanian Seed Conservation Centre established at the Royal Tasmanian Botanical Gardens is a central part of Tasmanian ex situ conservation efforts for ex situ conservation programs. An ongoing role for this facility is considered fundamental.